Fig. 4

Pattern of immunoreactivity to major hub genes. Immunohistochemical staining of conventional ameloblastoma (top panel), mouse ameloblastoma xenograft derived from EPAMCs also isolated from conventional ameloblastoma (middle panel) and OKC (lower panel). The stromal compartments (black star) of ameloblastoma and mouse ameloblastoma xenografts were strongly immunoreactive to FN1, IGF-1 and COL1 (top panel, columns 2,3 and 4). Similarly, stroma of mouse ameloblastoma xenograft (black star) was immunoreactive to FN1 and IGF-1 (middle panel, columns 2 and 3) but displayed minimal to no reactivity to COL I (middle panel, column 4). Similarly, the lower panel shows that stromal components (black star) of OKC immunoreacted with FN1, IGF-1 and COL 1 (lower panel, columns 2, 3 and 4). Additionally, OKC epithelial lining cells displayed (black arrows, column 2) immunoreactivity to FN-1. Comparative hematoxylin/eosin and negative antibody control tissue sections are shown in columns 1 and 5 respectively [Antibodies to FN1 = fibronectin 1; IGF-1 = insulin-like growth factor 1 and COL I = collagen 1. H&E = hematoxylin/eosin; control = non-immune serum; HA/TPC = hydroxyapatite/tricalcium phosphate carrier particles]