Table 1 The primer sequences
Gene | Primer Type | Sequence |
|---|---|---|
TCF7 | Forward (F) | 5’- ACC GCA ACC TGA AGA CAC AAG C-3’ |
Reverse (R) | 5’- CAA TGA CCT TGG CTC TCA TCT CCT TC-3’ | |
CTNNB1 | Forward (F) | 5’- TCT GAG GAC AAG CCA CAA GAT TAC AAG-3’ |
(β-catenin) | Reverse (R) | 5’- TCA GCA GTC TCA TTC CAA GCC ATT G-3’ |
WNT2B | Forward (F) | 5’- TCC GAG AGT GTC AGC ACC AAT TC-3’ |
Reverse (R) | 5’- GCG AGT AAT AGC GTG GAC TAC CC-3’ | |
LEF1 | Forward (F) | 5’- TCT TCC TTG GTG AAC GAG TCT GAA ATC-3’ |
Reverse (R) | 5’- GGC TCC TGA GAG GTT TGT GCT TG-3’ | |
GAPDH | Forward (F) | 5’- CAG GAG GCA TTG CTG ATG AT-3’ |
(reference gene) | Reverse (R) | 5’- GAA GGC TGG GGC TCA TTT-3’ |