Table 1 The materials used and the study groups in the study
Irrigation Agents | Chelating Technique | Representative Group Name | Concentration and pH | Total Volume | Contact Time |
|---|---|---|---|---|---|
Sodium hypochlorite | No chelator used | Control | 2.5% NaOCl (pH = 12) | NaOCl: 32 mL DW: 5 mL | NaOCl: 20 min (including instrumentation time). DW: 1 min |
Sodium hypochlorite / Ethylenediaminetetraacetic acid | Sequential chelation | EDTA | 17% EDTA (pH = 7.4) | NaOCl: 32 mL DW: 5 mL EDTA: 4 mL DW: 5 mL | NaOCl: 20 min (including instrumentation time). DW: 1 min EDTA: 2 min DW: 1 min |
Sodium hypochlorite / Etidronic acid | Sequential chelation | HEDP | 9% HEDP (pH = 2.4) | NaOCl: 32 mL DW: 5 mL HEDP: 4 mL DW: 5 mL | NaOCl: 20 min (including instrumentation time). DW: 1 min HEDP: 2 min DW: 1 min |
Sodium hypochlorite / Phytic acid | Sequential chelation | PA | 2.5% PA (pH = 0.9) | NaOCl: 32 mL DW: 5 mL PA: 4 mL DW: 5 mL | NaOCl: 20 min (including instrumentation time). DW: 1 min PA: 2 min DW: 1 min |
Etidronic acid and sodium hypochlorite mixture | Continuous chelation | DR HEDP | 9% DR HEDP (pH = 5.6) | HEDP + NaOCl: 32 mL DW: 5 mL | HEDP + NaOCl: 20 min (including instrumentation time). DW: 1 min |
Phytic acid and sodium hypochlorite mixture | Continuous chelation | DR PA | 2.5% DR PA (pH = 7.8) | PA + NaOCl: 32 mL DW: 5 mL | PA + NaOCl: 20 min (including instrumentation time). DW: 1 min |