Fig. 1

schematic diagram showing the study design. The pulp tissues were harvested from incisors’ rats followed by isolating DPCs that were ultracentrifuged to isolate DPCs-sEVs. The sEVs were characterized using transmission electron microscope (TEM), western blotting (WB), nano tracking assay (NTA) and bicinchoninic acid (BCA) assay. To evaluate the effect of DPCs-sEVs on DPCs, in comparison to MTA; MTT assay, transwell migration assay and RT-PCR were performed